Molecular Biology 3.1
Description
Advanced module on DNA isolation, manipulation, and analysis from the perspective of genetic engineering of biological and biopharmaceutical products.
Learning Outcomes
Describe the underlying theory of end-point and real-time PCR and their application in recombinant DNA technology.
Explain the role of gene cloning and DNA analysis in research investigations of transcript product, gene expression, gene regulation, and translation product identification.
Explain the role of gene cloning and DNA analysis in biotechnological applications, including commercial protein production, medicine, agriculture, and forensic science.
Use appropriate reagents and equipment to PCR amplify and sequence DNA.
Use appropriate reagents and equipment to study heterologous proteins.
Use appropriate reagents and equipment for DNA fragment analysis