Advanced Molecular and Biochemical Techniques
The main purpose of this module is to introduce the learner to theoretical and practical aspects of recombinant protein production and how this relates to upstream processing in the production of biopharmaceuticals. The module builds on molecular, microbiology and biochemistry modules in year 2 and prepares learners for more advances studies in biopharmaceutical technology and molecular biology in year 4.
Discuss homologous and heterologous recombinant protein expression with a focus on (but not limited to) protein expression in E. coli. Distinguish between other expression hosts including yeasts, mammalian cell lines, insect cell lines, transgenic plants and animals.
Evaluate and carry out a variety of protein quantification methods and protein analysis techniques such as SDS-PAGE and Western Blotting and bioinformatic analysis
Describe protein modification methods at both the DNA codon and amino acid levels.
Produce and analyse a recombinant protein.
Recognise how the production of a protein in a recombinant cell line relates to upstream processing in biopharmaceutical production.