This module introduces the learner to the theory of modern Chromatography. This includes the instrumentation and detectors associated with Gas Chromatography (GC), High Performance Liquid Chromatography (HPLC) and Ion Chromatography (IC) . This module also covers sample preparation and extraction with particular emphasis on trace analysis. The analysis of various pharmaceutical and forensic samples will be carried out in the laboratory to practically illustrate the theory and enable students to develop their analytical laboratory skills.
Understand the major differences between Gas Chromatography and HPLC. Be familiar with the associated technique of Ion Chromatography
Choose the correct chromatographic analytical technique and detector depending on the physical properties of the sample being analysed
Recognise when to use external standards, internal standards and standard addition procedures for calculating the content of unknown samples
Know how to extract samples using solvent-solvent extraction and solid phase micro extraction
Perform and interpret qualitative and quantitative chromatographic separations