This module introduces the learner to the theoretical basis and application of a variety of chromatographic equipment/techniques in chemical, biochemical, pharmaceutical and biopharmaceutical analyses. The analysis of various samples will be carried out in the laboratory to practically illustrate the theory and enable students to develop their analytical laboratory skills.
Discuss the underlying theoretical basis for and describe the operating principles of chromatographic techniques. Determine factors such as retention time (tR), adjusted retention time (tR'), Kav, Rf and resolution
Discuss the use of and applicability of most chromatographic methods in chemical, biochemical, pharmaceutical and biopharmaceutical analyses.
Operate a range of chromatography equipment and optimise separation, using known standards ( internal and external calibrants). Select the correct instrumental technique, most appropriate column type, most suitable standard and experimental conditions to maximise resolution.
Have some knowledge of sample pre-treatment to ensure maximum resolution and maximum column life.
Be able to apply these skills to the use of chromatographic methods in project Work/ industrial placement.